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학술발표회초록보기

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  • 02월 20일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제113회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Simultaneous Determination and Validation in Human Plasma by LC-MS/MS for Pharmacokinetics of Acetaminophen/Tramadol Combination Tablet

등록일
2014년 2월 19일 15시 33분 53초
접수번호
1306
발표코드
MEDI.P-895 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 16일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
의약화학
저자 및
공동저자
정영림
경기과학고등학교 화학생명과학부, Korea
A rapid, sensitive, and specific method for the simultaneous determination of acetaminophen and tramadol in human plasma, using acetaminophen-d4 and tramadol-d6 as internal standard, is described. Sample preparation involved a simple liquid-liquid extraction procedure. The extract was analyzed by high-performance liquid chromatography coupled to electrospray tandem mass spectrometry (LC-MS /MS). Chromatography was performed isocratically on a Hypersil gold C18 (2.1× 150 ㎜, 5 ㎛, Thermo) with acetonitrile and 10 mM ammonium acetate (pH 4.0) (50:50, v/v) as mobile phase. The instrument was set up in multiple reaction monitoring (MRM) mode; the transition m/z 152.1 → 110.1 was monitored for acetaminophen and the transition m/z 264.2 → 58.2 for tramadol. Calibration curves were linear over the range of 0.02∼10 ug/mL for acetaminophen and 0.5∼300 ng/mL for tramadol. The inter-run relative standard deviations were less than 6.37% for acetaminophen and 5.34% for tramadol. The intra-run relative standard deviations were less than 3.11% for acetaminophen and 2.47% for tramadol. The mean plasma extraction recovery to acetaminophen and tramadol were in the ranges of 71.54∼77.24% and 91.82∼98.39%. The developed assay method was applied to a pharmacokinetic study in healthy volunteers after oral administration of a new formulation acetaminophen/tramadol combination tablet.

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