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  • 02월 20일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제113회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Development of inhibitory peptide as an antifungal agent towards Phytophthora capsici microtuble

등록일
2014년 2월 20일 16시 41분 35초
접수번호
1413
발표코드
BIO.P-581 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 16일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
윤문영
한양대학교 화학과, Korea
Phytophthora blight of pepper caused by an oomycete pathogen Phytophthora capsici(P. capsici) and is responsible for major losses in pepper production and the pathogen can survive in soil in the absence of the host plant for many years. Microtubules have several key roles that are important in cell proliferation, trafficking, signaling and migration in eukaryotic cells. Due to these reasons microtubule is an attractive target of many antifungal agents. In this study, we screened alpha and beta tubulin binding peptides from Phytophthora capsici by phage display method. After five rounds of biopanning, we identified peptides showed picomolar range of high binding affinity from a random peptide library toward P. capsici alpha and beta tubulin. The specificity of peptides was found 78% higher compared to bacterial FtsZ from Xanthomonas axonopodis; which has similar role to that of eukaryotic tubulin. Among them, a couple of peptides showed high inhibition against microtubule formation with IC50 in the range of 890 nM ? 1.3 μM concentration which is much lower than well-known chemical inhibitor Benomyl (IC50 = 500 μM). Based on these results, it is anticipated that these peptides could further be used promising candidate as novel antifungal agent against Phytophthora blight disease.

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