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학술발표회초록보기

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  • 02월 20일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제113회 대한화학회 학술발표회, 총회 및 기기전시회 안내 STORM for live cells: super-resolution fluorescence microscopy via single-molecule localization

등록일
2014년 2월 26일 15시 01분 45초
접수번호
1438
발표코드
PHYS.O-4 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 45분
발표형식
구두발표
발표분야
물리화학 - General Oral Presentation
저자 및
공동저자
심상희
울산과학기술대학교(UNIST) 생명공학과, 화학과, Korea
“Super-resolution” fluorescence microscopy has overcome the diffraction limit of optical microscopes over an order of magnitude. One major group of such methods utilizes photoswitching probes for separating fluorophores in time, thereby localizing single molecules at high precision. This group was first introduced by the names of STORM (stochastic optical reconstruction microscopy) and PALM (photoactivated localization microscopy). STORM/PALM has demonstrated up to 10-nm resolution as well as multicolor and 3D capabilities using standard optical microscopes.
Here, I will present STORM imaging methods for living cells. Proteins in live cells were imaged with photoswitchable cyanine dyes whose brightness and fast switching enabled 3D resolutions of 30-50 nm within 1-2 seconds. Membranes in live cells were stained with small-molecule probes that allowed us to resolve previously obscured details on dynamics of the plasma membrane, the endoplasmic recticulum (ER) and mitochondria. The live-cell STORM methods open new windows for visualizing nanometer-scale dynamics in vivo.

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