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제113회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Localized Surface Plasmon Resonance (LSPR) Biosensors Based on Metal Nanoparticles with the Design of Bioreceptors

등록일
2014년 3월 4일 14시 14분 27초
접수번호
1443
발표코드
ANAL1-5 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 11시 : 10분
발표형식
심포지엄
발표분야
분석화학 - Advanced Bio and Environmental Analysis I: Sensor and Chip
저자 및
공동저자
김민곤
광주과학기술원(GIST) 화학과, Korea
Label-free biomolecular assay based localized surface plasmon resonance (LSPR) of noble metal nanoparticles enables simple and rapid detection with the use of simple equipment. Nanosized metal nanoparticles exhibit a strong absorption band when the incident light frequency is resonant with the collective oscillation of the electrons, which is known as the LSPR. Here we demonstrate localized surface plasmon resonance (LSPR) substrates such as plasmonic Au nanodisks fabricated by a nanoimprinting process and gold nanorod-immobilized surfaces and their applications to highly sensitive and/or label-free biosensing. To increase detection sensitivity various bioreceptors weree designed. A single chain variable fragment (scFv) was used as a receptor to bind C-reactive protein (CRP). The results of this effort showed that CRP in human serum could be quantitatively detected lower than 1 ng/ml. Aptamers, which were immobilized on gold nanorods, were used to detect mycotoxins. The specific binding of ochratoxin A (OTA) to the aptamer was monitored by the longitudinal wavelength shift of LSPR peak in the UV-Vis spectra resulting from the changes of local refractive index near the GNR surface induced by accumulation of OTA and G-quadruplex structure formation of the aptamer. According to our results, OTA could be quantitatively detected lower than 1 nM level. Additionally, aptamer-functionalized GNR substrate was quite robust and can be regenerated many times by rinsing at 70 OC to remove bound target. During seven times of washing steps, the developed OTA sensing system could be reusable. Moreover, the proposed biosensor exhibited selectivity over other mycotoxins with an excellent recovery for detection in grinded corn samples, suggesting that the proposed LSPR based aptasensor plays an important role in label-free detection of mycotoxins.

References
[1] Ju-Young Byun, Yong-Beom Shin, Taihua Li, Jin-Ho Park, Dong-Myung Kim, Dong-Hwan Choi, Min-Gon Kim* (2013) The use of an engineered single chain variable fragment in a localized surface plasmon resonance method for analysis of the C-reactive protein. Chem. Commun., 49 (82), 9497-9499
[2] Lee SW, Lee KS, Ahn J, Lee JJ, *, Kim MG*, Shin YB (2011) Highly sensitive biosensing using arrays of plasmonic au nanodisks realized by nanoimprint lithography. ACS Nano, 5(2):897-904.

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