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  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Enzyme linked aptamer assay for the determination of Tetracycline residue in foods

2014년 8월 18일 14시 43분 41초
ANAL.P-495 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 15일 (수요일) 16:00~19:00
저자 및
한수정, 이인숙*
서울여자대학교 화학과, Korea
Enzyme-linked immunosorbent assay (ELISA) is the most commonly used tool for protein detection and quantification in complex solutions. Here we applied oligonucleotide, aptamer as a capture binder based on avidin/biotin interaction on multi-well polystyrene plates, and three different oligonucleotide [prove 1, 2 and 3] as a detecting binder, in its standard "sandwich" set up. Tetracyclines (TCs) are a group of broad-spectrum antibiotics which prevent and control many bacterial infectious diseases. So, they are widely used to treat many infections and growth promotion in animal husbandry. However, an abuse of antibiotics can increase the risk of TCs remaining in foods and cause serious problems on drug tolerance. To ensure the safety of food, it is necessary to establish a reliable analytical technique with specificity and sensitivity. This work represents enzyme linked aptamer assay (ELAA) by sandwich design for the determination of TC residue in foods. Aptamers are functional single-stranded oligonucleotides (ssDNA or RNA), which are selected in vitro by SELEX process. And they binds to a specific target molecule with high affinity and selectivity, from small molecules to proteins. A 76mer DNA TC aptamer was utilized as capture and three complementary oligonucleotides (36mer-ssDNA, 37mer-ssDNA and 76mer-ssDNA) were used as detector. Various assay conditions were optimized and a proposed assay design is expected to become a promising method for the determination of TCs in contaminated food product. From those results, a binding site of 76mer-DNA TC aptamer also could be identified indirectly.