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  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Comparative study of oxidized phospholipids in lipoprotein classes between controls and patients with coronary artery disease by flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry

등록일
2014년 8월 26일 17시 35분 13초
접수번호
0308
발표코드
ANAL.P-503 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
이주용, 문명희*
연세대학교 화학과, Korea
Since several decades ago, lipoprotein particles in blood that transports lipids, has been treated as one of the most critical topics in terms of coronary artery disease (CAD). Lipoproteins can be classified into three classes by their densities and specific roles they play. High density lipoprotein (HDL) transports lipids from body to liver for decomposition of the fats. Very low density lipoprotein (VLDL) is synthesized in liver for storing the remaining fat, and low density lipoprotein (LDL) is formed by loss of TGs from the VLDL. When excess amount of lipoproteins build up on blood vessels, they are oxidized by either non-enzymatic or enzymatic processes and start to malfunction. According to recent clinical studies, the oxidized lipoproteins are critical biomarkers of CAD because they promote the formation of the plaque on blood vessels. Studying oxidized phospholipids (PLs) within the oxidized lipoprotein is the main objective of this study. Due to the capability of increased amount of injection and disposability, a multiplexed hollow-fiber flow field-flow fractionation (MxHF5) system was applied for the separation and collection of lipoprotein particles from blood plasma. HDL, LDL and VLDL from two groups of pooled blood plasma samples, 10 controls and 10 patients with CAD, separately, were successfully separated by MxHF5 system without any pre-treatment. Nanoflow liquid chromatography-tandem mass spectrometry was used to identify the oxidized phospholipids from the collected lipoprotein classes of blood plasma. Oxidation, peroxidation and cleavage of fatty acid from PLs were successfully profiled from the fragmentation patterns of collision-induced dissociation and comparative analysis of oxidized PLs between controls and patients were carried out.

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