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학술발표회초록보기

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  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Rapid Detection of DNA by Magnetophoretic Assay

등록일
2014년 8월 27일 14시 47분 48초
접수번호
0461
발표코드
ANAL.P-519 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
주굉건, ZOUFENGMING1, 고광락2, 이재범3,*
부산대학교 나노융합기술대학, China
1부산대학교 나노과학기술대학, China
2부산대학교 나노과학기술대학, Korea
3부산대학교 나노메디컬공학과, Korea
Recently, marine environmental pollution has become more and more serious due to oil spills or the collapse of nuclear power plant resulting in a massive release of hazardous materials to the ocean. These harmful radioactive materials are associated with the random mutation of genes leading to deformity of individuals. So far there has been no effective system for monitoring this marine pollution. Therefore, rapid detection of DNA mutations has become increasingly important in a variety of fields including medical diagnostics and food safety. Herein, we report a rapid, sensitive, qualitative and quantitative approach for DNA detection based on the magnetophoretic assay. The Au-magnetic microparticles (MMPs) nanocomplex was used to generate a color signal and uncombined Au NPs contributed an optical signal that could be directly quantified by UV-Vis absorption spectroscopy of the supernatant after the sample was placed in a magnetic field for 2 min. The linear relationship between signal intensity and the target DNA concentrations (0.1 nM ~ 1.0 ?M) was investigated and a limit of quantitation of 0.1 nM was achieved using this method.

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