Peroxynitrite has a multiple physiological and pathological roles in cellular signaling transduction and cell damage. Therefore, the interest in developing fluorescent probe for selective detection of this reactive oxygen species has been increased these days.
The red emitting probe CHCN can dedect ONOO- and CHCN is dissapeared in the presence of ONOO- and be replaced by green emission.
ONOO- produced by an oxidation process reacts with CHCN and shows a dual ratiometric and colorimetric response.
CHCN has appreciable cell permeability and, as a result, it is applicable to ratiometric detection of exogenous and endogenous ONOO- in living cells during phagocytic immune response.
Therefore, We anticipate that, owing to their ideal properties, we can look cell imaging easily and this type of probe will find great use in explorations of the role played by ONOO- in biology.
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