Nano materials have been explored for various applications owing to their significant properties when compared with the bulk materials. Iron oxide nanoparticles (Nano Fe3O4), belongs to the class of metal oxide that is well known from ancient times. Carbon based nanomaterials, specifically carbon nanotubes are another interesting class of nanomaterial with unique properties like high surface area, superior mechanical strength, good electrical conductivity, high thermal conductivity, etc. Hence, they find applications in various diverse fields such as chemical & bio-sensing, fuel cells, solar cells, batteries, energy storage, electronic applications, etc.
Highly sensitive and selective enzyme based biosensors have gained immense popularity due to their high specificity and high turnover numbers. Generally, redox mediators are used to improve the sensitivity and shorter the response time of the sensor but, it causes interferences. The incorporation of an appropriate nanointerface at the electrode can aid in faster transfer of electrons. Such superior sensitivity and specificity will be invaluable in assessing food quality and as disease diagnosis in the healthcare sector. In the case of food industries, the determination of hydrogen peroxide in milk can indicate the associated health risk while, the determination of freshness of fish can be utilized for the export industries. In the health care sector, detection of superoxide in biological samples can serve to diagnose various oxidative stress related disorders. The current topic of my presentation is based on the development of suitable nanointerfaced electrochemical biosensors for testing the quality of milk & fish and the clinical biomarker, superoxide.