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  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Development of home-built solid-state NMR probes for particular purposes

2014년 8월 28일 17시 01분 24초
ANAL.P-578 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 15일 (수요일) 16:00~19:00
저자 및
정지호, 최성섭, 김용애*
한국외국어대학교 화학과, Korea
The solid-state NMR has been widely used for many insoluble materials like industrial polymers and biological samples. Also solid-state NMR analysis of membrane protein on lipid bilayer or bicelle as a membrane-like environments, is especially valuable for their structure and function relationship. However, most of biological proteins on lipid bilayers have high dielectric property due to containing large amounts of lipids, water, and salts as well as probe efficiency are decreased cause of their electrical properties of lossy samples. Therefore, specific probe with high efficiency is required to study biological samples by using solid-state NMR. Development of solid-state NMR methodologies for the study of orientational information of membrane protein in bicelle could be used for the in-situ analysis of liquid crystalline materials in LCD panels as well as Li materials in Li ion batteries. Here, we present the optimized design, construction, and efficiency of a home-built 400 MHz wide-bore 1H-15N solid-state NMR probe with 5-mm solenoidal rf coil and a home-built 800 MHz narrow-bore 1H-15N solid-state NMR probe with strip-shield coil to prevent heating by high RF power for the study with lossy samples. 1H-15N 2D SAMPI4 spectra from a single crystal and membrane proteins oriented in bicelles were successfully obtained by using these solid-state NMR probe. Also, we present a home-built 500 MHz NB 19F-13C double resonance solid-state NMR probe with a flat-square coil and 600 MHz NB 19F-7Li double resonance solid-state NMR probe with a solenoidal coil. These were the first application for the in-situ analysis of LCD panel and in-situ analysis of Li ion battery pack samples. These probes provide short pulses, high power capability, and good RF homogeneity.