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  • 09월 04일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제114회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Technical Developments of Correlative Microscopy using Transient Receptor Potential Canonical (TRPC) 4 and Quantum dot in vivo

2014년 9월 4일 15시 02분 03초
BIO.P-656 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 15일 (수요일) 16:00~19:00
저자 및
석민숙, 전상미1,*
한국기초과학지원연구원 전자현미경부, Korea
1한국기초과학지원연구원 전자현미경연구부, Korea
The trasient receptor potential (TRP) channel family is a class of Ca2+ permeable channels. TRPC4 is a member of the transient receptor potential canonical channels. This protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Although the extensive studies on the function of TRPC4 have been reported so far, the mechanisms of Calcium sensing and signaling through the transmembrane region are remains elusive. We have shows that with the use of immunohistochemistry, Quantum dot express TRPC4 at the cell membrane. By confocal imaging and Cryo-electron microscopy, we detected localization and changes of a few fluorescence signals upon binding of the Calcium in cells of TRPC4. These results may provide valuable insights for the further structural study of transmembrane domain of TRPC to elucidate the activation mechanism. Therefore, further structural studies of TRPC4 in cells with various labeling strategies and Cryo-electron tomography (CryoET) allows 3D visualization of cellular will be needed. Furthermore, to enable imaging of frozenhydrated and fluorescently labeled biological specimens on EM grids, we have developed a homebuilt cryo-fluorescence light microscopy stage. This allows direct correlation between two sets of imaging data, optical imaging and electron imaging. The correlative approach detailed here will be valuable for broader applications in cell biology, such as cell signaling, membrane receptor trafficking, and many other dynamic cellular processes.