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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Real-time Dynamic Super-Resolution Imaging of Mg2+ Signaling and Wave Propagation by Direct Stochastic Optical Reconstruction Microscopy

2015년 2월 23일 16시 04분 08초
KCS2.O-3 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 09시 : 30분
Oral Presentation for Young International Chemists
저자 및
Chakkarapani Suresh Kumar, 강성호1,*
경희대학교 화학과, Korea
1경희대학교 응용화학과, Korea
Magnesium ion (Mg2+) signaling and dynamics in wave propagation were monitored and imaged by direct stochastic optical reconstruction microscopy (dSTORM). dSTORM, a super-resolution technique with an increased spatial resolution have the potential to monitor and image the intracellular organization of the cell with sub-nanometer resolution. In dSTORM, fluorophores were photoswitched between fluorescent on state and fluorescent off state by simultaneous irradiation of an activation laser and an excitation laser in the presence of a photoswitching buffer. Mag-fluo-4-AM, an intracellular fluorescent indicator dye was incubated in human embryonic kidney 293 (HEK-293) and photoswitched to super-localize the position of Mg2+ with subdiffraction-limit resolution. The resulting point spread function measured from activated fluorophores was analyzed by applying Guassian fitting. Further, Na+ATP was added along with mag-fluo-4-AM to monitor and image the wave propagation of Mg2+ in the membrane sites of HEK-293 cell. The real-time dynamics in wave propagation was observed at 0.2 s time interval with spatial resolution of around 20 nm. The results suggest that the intracellular Mg2+ signaling and wave propagation was imaged with sub-nanometer spatial resolution and lower temporal resolution of 0.2 s