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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Probing protein interactions and dynamics in a single living cell

등록일
2015년 2월 25일 13시 44분 12초
접수번호
1331
발표코드
PHYS1-5 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 11시 : 10분
발표형식
심포지엄
발표분야
물리화학 - Biophysical Chemistry: Biological Phenomena Viewed by Physical Chemistry
저자 및
공동저자
이남기
포항공과대학교(POSTECH) 시스템생명공학부/물리학, Korea
Dynamic interactions between membrane proteins and ligands determine correct cellular responses to diverse environmental changes. Because various protein complexes on a plasma membrane participate in cellular functions, technique identifying complex interactions of membrane proteins with spatio-temporal resolution in a single living cell is highly demanding. Here I present an imaging technique of membrane protein interactions that utilizes the accurate measurement of diffusion coefficient of membrane proteins by tracking individual proteins in a single living cell. We validated this approach that can measure the interactions between ligands and membrane proteins using an epidermal growth factor receptor and show its applicability in measuring dissociation constant of ligands and mutant specific interactions in a single living cell. We extended our technique to G protein-coupled receptors, which confirms the dissociation of Gi-protein at the downstream of ligand interactions. Our results demonstrate that our approach would be a powerful technique for investigating the various interactions of membrane proteins and the processes of signal transduction in a single living cell.

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