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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A Microfluidic Chip based Strategy for Biopharmaceutical Glycosyl Modification Analysis

등록일
2015년 2월 25일 15시 17분 51초
접수번호
1338
발표코드
ANAL.P-581 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
서영숙, 안현주*
충남대학교 분석과학기술대학원, Korea
Glycosylation plays an important role in ensuring the proper structure and function of most biotherapeutic proteins. However, bioactive glycan modifications add significant complexity to an already-diverse biopharmaceutical glycome. This diversity can greatly complicate glyco-analytical efforts during drug development, production, and regulatory approval. To address this issue, we have developed a novel microfluidic chip capable of online capture, enrichment, and LC separation of phosphoglycans. The chip allows online fractionation of phosphoglycans and non-phosphoglycans onto two different chromatographic runs, with LC gradients and instrumental settings independently optimized for each. Analysis is initiated by injection of glycan mixture onto the two-layer enrichment column. Phosphoglycans are trapped and enriched by the titanium dioxide(TiO2) layer of the enrichment column, while non-phosphoglycans flow past the TiO2 layer and are trapped by the PGC layer of the enrichment column. Following enrichment, a water/acetonitrile gradient elutes the non-phosphoglycans from the PGC layer of the enrichment column, which further separates neutral and sialylated glycans. Next, phosphoglycans are eluted from the TiO2 layer of the enrichment column by injection of a high-pH aqueous elution buffer. We analyzed phosphoglycans selectively by LC/MS chip with a TiO2-PGC enrichment column. This chip-based strategy for biopharmaceutical glycan analysis increases chromatographic peak capacity, enhances MS sensitivity, and improves glyco-analytical capabilities.

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