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현재 가능한 작업은 아래와 같습니다.
  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Digital Microfluidics as a Versatile Bioanalysis Tool

2015년 2월 26일 04시 22분 24초
ANAL2-5 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 15시 : 20분
분석화학 - Recent Trends in Separation Analysis II: Applications
저자 및
한국표준과학연구원(KRISS) 유기분석표준센터, Korea
Digital microfluidics (DMF) is a fluid handling technique in which discrete droplets are manipulated on the surface of an array of electrodes. In this talk, I will present two recent projects using solid samples on a DMF device. First, I will describe the work integrating solid-phase microextraction (SPME) in DMF. In the new system, a SPME fiber is used to extract analytes from a complex sample, and is then inserted into a DMF device. Solvent droplets are then used to extract analytes from the fiber with continuous actuation, followed by analysis with liquid chromatography and mass spectrometry. Compared to conventional methods, the new technique allows for fast, efficient desorption of analytes from the SPME fiber using a small volume of solvent. The miniaturized system allows for preconcentration of analytes and was applied the analysis of steroid hormones in human urine. Second, I will describe our work implementing magnetic particle-based immunoassays in DMF. To increase the assay throughput, a new platform capable of performing complete immunoassays with minimal manual intervention was developed. This instrument comprises three core components: a Pogo pin interface for digital microfluidic control, an integrated photomultiplier tube for chemiluminescent detection, and an adjustable magnet for particle separation. Using this automation system allowed for parallel sample processing and full factorial design of experiments (DOE) optimization. Relative to macroscale methods, this DMF approach reduced reagent volumes and analysis time, and moreover, this represents the first DOE optimization for immunoassays implemented in a microfluidic system. I propose that these techniques have great potential for numerous applications in bioanalysis and beyond.