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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Development of inhibitory aptamer against Mycobacterium tuberculosis based on inhibition of acetohydroxyacid synthase

등록일
2015년 2월 26일 09시 30분 19초
접수번호
1371
발표코드
BIO.P-645 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
문지영, 윤문영*
한양대학교 화학과, Korea
Acetohydroxyacid synthase (AHAS) in Mycobacterium tuberculosis (MTB) is a promising and potential target for the development of new anti-tuberculosis agents. AHAS from MTB is one of the biosynthetic enzymes, which catalyzes the first common step in the biosynthesis of the essential branched chain amino acids (BCAA’s: valine, leucine, and isoleucine). We identified short (30mer) single stranded DNA aptamers as novel inhibitor against MTB AHAS through systematic evolution of ligands by exponential enrichment (SELEX). Total 9 aptamers were identified with strong and specific binding to MTB AHAS. Among identified 9 aptamers, 2 aptamers (Apt 1, Apt 6) are the most potent inhibitors against MTB AHAS with IC50 in the low nanomolar range (28.94 and 22.35, respectively). These two aptamers were modified and changed as minimal inhibitory aptamers. Apt1-17mer and Apt6-20mer were evaluated for In-vitro test and exhibited strong inhibition against multi drug-resistant and extensive drug-resistant MTB strain with low MIC 5.27 μg/ml and 6.16 μg/ml. Taken together, results of this study provide impetus for the development of strong anti-tuberculosis agents and may overcome the issue of drug resistance.

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