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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Screening of high-affinity ssDNA aptamers against dihydrostreptomycin

등록일
2015년 2월 26일 09시 38분 44초
접수번호
1373
발표코드
BIO.P-647 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
하나름, 윤문영*
한양대학교 화학과, Korea
Dihydrostreptomycin is an aminoglycoside antibiotic consisting of a hydrogenated form of streptomycin, which is usually used in vetinary medicine in cattle, pigs, chicken and sheep. The use of antibiotics to treat food animals has the potential to affect human health. The risk of antibiotic residues in meat and milk is well known and focuses on intensive regulatory processes. Thus it is necessary to develop highly sensitive diagnostic kit for residual antibiotics. In this study, we screened the ssDNA aptamers against dihydrostreptomycin. We used the DNA binding plate for fixation of target and screening. We determined the saturation conditions of target binding on the well surface and was found to be 5mM of dihydrostreptomycin. Total 11 rounds of SELEX was progressed including negative selection for kanamycin, streptomycin and we obtained aptamers. These aptamers were synthesized and characterized with respect to their Kd value and specificity using aptamer-based ELISA method. Furthermore, aptamer conjugation with polydiacetylene (PDA) will be discussed in order to development of aptasensor based on colorimetric system for detection of antibiotics.

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