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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Development of Novel Hab mutases from database search

등록일
2015년 2월 26일 14시 42분 31초
접수번호
1444
발표코드
ORGN.P-903 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
유기화학
저자 및
공동저자
조새롬, 이혁1,*
과학기술연합대학원대학교(UST) 의약 및 약품화학, Korea
1한국화학연구원 의약화학 연구 센터, Korea
Biotransformation using enzymes or whole-cell catalysts has been emerging as one of major synthetic tools in organic synthesis. As a biocatalyst, Hab mutase has been known to convert hydroxylaminobenzene to aminophenol regioselectively. Recently we have found a novel Hab mutase through High-throughput screening of metagenomics libraries, and developed a new synthetic pathway from nitrobenzenes to aminophenols. Based on this result, database search using BLASTp found 62 organisms that have similar sequence to the original HabM. Among these 62 Hab Mutase-like proteins, 30 proteins with 25-80% sequence identity were selected and phylogenetically compared using Clustal W2. Finally, 17 Hab Mutase-like proteins with least sequence similarity among each other were selected, and their gene synthesized for recombinant expression in Escherichia coli. Then we checked Hab Mutase activities of those proteins by testing the biotransformation of hydroxylaminobenzene to 2-aminophenol, and 5 novel Hab Mutases were identified.

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