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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Label-free Impedimetric Polytetrabenzidene/Dendrimer Nanocomposite Based Highly Sensitive Immunosensor: Femtomolar Detection of Cardiac Troponin I in Serum

등록일
2015년 2월 26일 16시 45분 46초
접수번호
1505
발표코드
ANAL.P-586 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 15일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
Akter Rashida, 정봉진, Md. Aminur Rahman*
충남대학교 분석과학기술대학원, Korea
A simple and robust but highly sensitive label-free impedimetric cardiac troponin I (CTI) immunosensor was fabricated using a poly-3, 3, 5, 5-tetramethylbenzidine (PolyTMB)/dendrimer (Den) nanoassemble film as anti-CTI antibody immobilizing platform. PolyTMB was electrochemically grown on a gold (Au) electrode by a potential cycling method. Carboxylic acid functionalized third generation poly(amidoamine) dendrimer (Den) and protein A (PA) was then sequentially covalently attached on the PolyTMB and PolyTMB/Den films, respectively. Anti-CTI antibody was then covalently immobilized on the PolyTMB/Den film with controlled orientation. The surface of the immunosensor was characterized using electrochemical quartz crystal microbalance (EQCM), coulometry, scanning electron microscopy (SEM), Fourier Transform Infrared (FT-IR) spectroscopy. The highly sensitive CTI detection was made by monitoring the impedance changes of the immunosensor surface due the anti-CTI-CTI binding, which affect the PolyTMB redox activity. CTI could be selectively detected at a fg/mL level in human serum samples.

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