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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Two-Photon Fluorescent Probes for Monitoring Intracellular Zinc Ions

등록일
2015년 2월 26일 18시 11분 36초
접수번호
1521
발표코드
KCS2.O-4 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 09시 : 45분
발표형식
구두발표
발표분야
Oral Presentation for Young International Chemists
저자 및
공동저자
Hardev Singh Virdi, 김환명*
아주대학교 에너지시스템학부, Korea

Among the various transition metals ions, zinc is the most abundant ion in the human body1. It is widely found in proteins where it plays important role in various biological processes. The biological imbalance in its concentration has been linked to many neurological disorders such as Alzheimer’s and Parkinson’s diseases2. Recently, the zinc ions have been regarded as quantitative imaging biomarker for the early detection of prostate cancer3. Out of the total Zn2+ ion (approximately 0.2 mM) content in mammalian cells, fee Zn2+ concentration is as low as picomolar to nanomolar levels. A number of one photon fluorescent probes for the detection of zinc ions have been developed and investigated4. However these probes have certain limitations as they requires a rather short excitation wavelength that limits their use in tissue imaging up to deep penetration depth (<100 μm), photo-bleaching and cellular auto-fluorescence. Realizing the importance of zinc in biological cell and limitation of using one photon microscopy, we have design and synthesized two-photon fluorescent probes SZn1 and SZn1-Acid, which consist of two naphthalene-derived fluorophore unit and a methoxy derivative of N,N-di-(2-picolyl)ethylenediamine as the Zn2+ chelator. Both derivatives show the dramatic increase in their fluorescence intensities upon the addition of Zn2+ ions. Further the good water solubility, high selectivity, Kd values in nanomolar range and insensitivity to biological relevant pH range make them suitable candidate for monitoring the zinc ions in live cells and tissues. In the oral presentation, the synthesis, photo-physical behaviour and bio-imaging applications of these probes will be discussed.

References
1. Frederickson, C. J.; Koh, J. Y.; Bush, A. I. Nat. Rev. Neurosci. 2005, 6, 449.
2. (a) Bush, A. I. Curr. Opin. Chem. Biol. 2000, 4, 184. (b) Bush, A. I. Trends Neurosci. 2003, 26, 207.
3. Ghosh, S. K.; Kim, P.; Zhang, X. A.; Yun, S. H.; Moore, A.; Lippard, S. J.; Medarova, Z. Cancer Res. 2010, 70, 6119.
4. (a) Sensi, S. L.; Ton-That, D.; Weiss, J. H.; Rothe, A.; Gee, K. R. Cell Calcium 2003, 34, 281. (b) A Guide to Fluorescent Probes and Labeling Technologies, 10th ed.; Haugland, R. P., Ed.; Molecular Probes: Eugene, OR, 2005.


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