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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Chemical Speciation Applied Supercritical Fluid Chromatography-atmospheric Pressure Photon Ionization Hydrogen/Deuterium Exchange (HDX) Mass Spectrometry for Structural Anaylsis

2015년 2월 11일 18시 32분 26초
ANAL.O-24 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 46분
분석화학 - Oral Presentation of Young Analytical Chemists
저자 및
조윤주, 김성환*
경북대학교 화학과, Korea
For comprehensive complicated mixture, structural identification is a very important for mass spectrometry analysis of poly aromatic hydrocarbon compounds. Hydrogen/Deuterium Exchange (HDX) technique has been used for structual interpretation in proteins, peptides, metabolites, antibiotics and petroleomics. However, HDX technique is limited because expensive deuterium solvent is used a lot and hence coupling to high performance liquid chromatography (HPLC) has been limited. In this study, HDX tendency of 21 standard compounds including polar heteroatoms at the molecular level was identified by combination of HDX in the process of atmospheric pressure photo ionization (APPI) and Supercritical fluid chromatography-Mass Spectrometry (SFC-MS). SFC utilizes supercritical carbon dioxide, methanol as mobile phase and offers a faster runtime in comparison with HPLC which is used aprotic solvents. In other words, mobile phase only a little of deuterated methanol is needed when HDX is applied to SFC. 0.3 mL of CH3OD for deuterium exchange was just used during all chemicals was eluted in 9 minutes. Overall, HDX SFC-MS was successfully applied to nitrogen-containing compounds preliminary to analysis of heavy crude oils for structual elucidation. Therfore, these results are examples of using powerful separation technique and we expect that this developed technique will be carried out to study crude oils and metabolites where a many unknown compounds have to be determined.