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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Carboxyl Modification of Peptides with Linear Aliphatic Diamines for Increasing Charge States

2015년 2월 11일 23시 47분 02초
ANAL.O-27 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 52분
분석화학 - Oral Presentation of Young Analytical Chemists
저자 및
김상진, 김한별, 서현경, 이용일, 신동수, 임재민*
창원대학교 화학과, Korea
Mass spectrometry has been used usually in protein identification. Especially mass spectrometry played a significant role in the analysis of peptides sequence, as well as posttranslational modification (PTM) analysis. However, analysis of PTMs, such as phosphorylation and glycosylation, is difficult with collision-induced dissociation (CID) by the loss of modification residues. The CID and electron-transfer dissociation (ETD) are complementary each other to interpret the PTMs. But ETD is particularly ineffective for the dissociation of tryptic peptide in doubly and singly charge states. In this study, we have tested carboxyl modification of peptides as a method for increasing the charge states of peptides in electrospray ionization with tandem mass spectrometry (ESI-MS/MS). The carboxyl-reactive crosslinker reagent 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate (HATU) is used to crosslink the carboxylic acid group of the peptide angiotensin II and the tryptic digested albumin peptides to linear aliphatic diamines such as ethane-1,2-diamine, butane-1,4-diamine, and hexane-1,6-diamine. As a result, the carboxyl modified peptides increased the most abundant charge state compared to native peptides. The longer crosslinkers effectively increased the most abundant charge state because of the reduction of the charge repulsion in the peptides. Key Words Peptide, Carboxyl modification, ETD, Mass spectrometry, HATU, and Linear aliphatic diamines