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학술발표회초록보기

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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 TEMPO-assisted FRIPS MS analysis of a peptide with three or more disulfide bonds

등록일
2015년 2월 12일 02시 07분 51초
접수번호
2030
발표코드
ANAL.O-30 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 58분
발표형식
구두발표
발표분야
분석화학 - Oral Presentation of Young Analytical Chemists
저자 및
공동저자
김은희, 오한빈*
서강대학교 화학과, Korea
We characterized the small proteins of insulin (51 amino acids, 5,733 Da) using FRIPS (free radical initiated peptide sequencing) mass spectrometry. Insulin consists of two segments, A-chain and B-chain, which are linked together by a multiple disulfide bonds, TEMPO-assisted FRIPS MS approach is reported to selectively cleave the disulfide bond. However, it is not reported yet whether or not it is applicable for peptides/proteins with multiple disulfide bonds. In the present study, we applied the TEMPO-assisted FRIPS approach to the insulin to see how multiple disulfide bonds affects the fragmentation behavior of peptides with multiple disulfide bonds. In our preliminary results, it was found that with TEMPO-assisted FRIPS can provide more detailed structural information of insulin without the reduction of disulfide bonds than the conventional CID could do. In the symposium, we will discuss the potential use future direction of the current research.

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