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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Radical-based peptide fragmentation MS

2015년 2월 12일 02시 10분 03초
ANAL.O-31 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 11시 : 00분
분석화학 - Oral Presentation of Young Analytical Chemists
저자 및
류이슬, 오한빈*
서강대학교 화학과, Korea
The bottom-up MS approach is most widely applied to identify and characterize proteins. However, there exists a weakness that the sequence coverage is rather low. To achieve a better sequence coverage, the middle-down MS approach has been suggested as a complementary tool, which is run in parallel with the bottom-up MS. With this information, we have sought to extend the TEMPO-assisted FRIPS (free radical initiated peptide sequencing) MS approach to the middle-down MS of proteins. As a preliminary study, first, we have explored the possibility of characterizing a relatively large peptide of melittin of ~3 kDa using TEMPO-assisted FRIPS MS, which showed extensive backbone fragmentations. Motivated at the promising results for melittin analysis, we applied the FRIPS methods to peptides produced by Lys-N digestion of BSA. As Lys-N cleaves at N-terminal side of lysine amino acid, it is expected that a longer peptide sequence can be obtained compared with the trypsin digestion that are mostly used in the bottom-up method. For the longer peptides, it was revealed that extensive a, c, x, and z-type fragments were generated in FRIPS MS. Further details of our study will be presented in the symposium.