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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Micro-headspace in-tube microextraction coupled with capillary electrophoresis

2015년 2월 12일 12시 43분 22초
ANAL.O-34 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 11시 : 06분
분석화학 - Oral Presentation of Young Analytical Chemists
저자 및
조성민, 이인기, 정두수*
서울대학교 화학부, Korea
In conventional headspace (HS) extraction, analytes evaporated from a sample donor phase in a sealed vessel can be enriched to an acceptor phase often in a liquid form or a sorbent coated on a fiber. The maximum enrichment factor is given by the ratio of the donor and acceptor volumes. Thus an acceptor of small volume is preferred and a single acceptor drop of small volume hanging to the capillary tip has been used in HS extraction for capillary electrophoresis (CE) [1]. To solve the stability problem of the hanging drop, we recently developed HS in-tube microextraction (ITME)/CE using a liquid plug inside a separation capillary as an acceptor in place of the drop [2]. Here, we present micro-headspace (?HS) ITME/CE, a simple but easy way of conducting HS extraction of a small volume sample for subsequent CE analysis. Instead of using a sealed vessel to prepare a HS, a ?HS of a volume much smaller than 1 ?L was formed at the tip of a capillary by injecting a plug of air into the capillary containing an acceptor plug and ejecting the air plug after immersing the capillary into a sample donor. Futhermore, ?HS-ITME-CE was performed using the acceptor plug remained in the capillary. Due to the small volumes of the ?HS and acceptor plug, it was possible even for a small volume donor to retain a high ratio of the donor and acceptor volumes for high enrichment factors. All steps of ?HS-ITME-CE were carried out automatically using a commercial CE instrument without any modification. Therefore not only the sample volume could be dramatically reduced, but also the need for a special apparatus was eliminated.