초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Development of home-built solid-state NMR probes for specific purposes

2015년 2월 12일 14시 41분 17초
ANAL.O-39 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 11시 : 16분
분석화학 - Oral Presentation of Young Analytical Chemists
저자 및
최성섭, 김용애*
한국외국어대학교 화학과, Korea
The solid-state NMR spectroscopy has been widely used for various insoluble materials like industrial polymers and biological samples. Also, solid-state NMR analysis of membrane protein on a membrane-like environments, such as lipid bilayer and bicelle, is especially valuable for their structure and function relationship. However, most of biological proteins on lipid bilayers have high dielectric property due to containing large amounts of lipids, water, and salts as well as probe efficiency are decreased cause of their electrical properties of lossy samples. Therefore, specific probe with high efficiency is required to study biological samples by using solid-state NMR. Also solid-state NMR methodologies for structural study of membrane protein in bicelle could be used for the in-situ analysis of liquid crystalline materials in LCD panels as well as Li materials in Li ion batteries. Here, we present the optimized design, construction and efficiency of a home-built 400 MHz wide-bore 1H-15N solid-state NMR probe with 5-mm solenoidal rf coil and a home-built 800 MHz narrow-bore 1H-15N solid-state NMR probe with strip-shield coil for structural study of membrane protein in bicelle. The 1H-15N 2D SAMPI-4 spectra from a single crystal and membrane proteins oriented in bicelles were successfully obtained by using these solid-state NMR probes. We also present the development of a home-built 500 MHz NB 19F-13C double resonance solid-state NMR probe with a flat-square coil and 600 MHz NB 19F-7Li double resonance solid-state NMR probe with a solenoidal coil for analysis of industrial materials. These were the first application for the in-situ analysis of LCD panel and in-situ analysis of Li ion battery pack samples. These probes provide short pulses, high power capability, and good RF homogeneity.