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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Differentiation of Ganglioside Isomers by MALDI TOF/TOF Mass Spectrometry

2015년 2월 13일 10시 08분 25초
ANAL.O-45 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 11시 : 28분
분석화학 - Oral Presentation of Young Analytical Chemists
저자 및
박우진, 이동건, 차상원*
한국외국어대학교 화학과, Korea
Gangliosides, acidic glycosphingolipids, have various structural isomers of which sialic acid residue positions are different from each other. Most abundant ganglioside isomers in biological systems are GD1a and GD1b, gangliosides with two sialic acids. In this study, we developed an effective matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) protocol for differentiating GD1a and GD1b isomers. For this purpose, we employed 5-methoxysalicylic acid (5-MSA) as a MALDI matrix because our recent study showed that this matrix induced minimal in-source fragmentation in the ionization of the labile gangliosides, GD1a. In addition, we incorporated salt additives of which cation charge state was +2 in the MALDI sample preparation because this cation could still carry +1 charge after charge neutralization of carboxyl anion group in a sialic acid residue and therefore more information-rich fragment ions could be generated in tandem MS experiments. Our results showed that relative intensities of isomer specific fragment ions were greatly enhanced in tandem mass spectra of GD1a and GD1b by employing doubly-charged cation such as calcium ion instead of singly-charged cation such as potassium ion.