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  • 02월 26일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제115회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Highly Sensitive Detection of Estradiol in Human Serum Using SERS-based Competitive Immunoassay

2015년 2월 23일 21시 10분 32초
ANAL.O-47 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 11시 : 32분
분석화학 - Oral Presentation of Young Analytical Chemists
저자 및
WANGRUI, 주재범1,*
한양대학교 바이오나노학과, Korea
1한양대학교 생명나노공학과, Korea
Estradiol (E2) is a biologically active sex steroid hormone that serves as important diagnostic marker in serum of premenopausal and postmenopausal women and in men. Therefore, it is very meaningful to establish highly sensitive and rapid methods for the detection of E2 in human serum. In the past decades, several technologies, including high performance liquid chromatography-mass spectrometry (HPLC-MS), gas chromatography-mass spectrometry (GC-MS) and immunoassays have been developed for the measurement of E2. However, the chromatography methods have lower sensitivity, and need well-trained personnel. Thus, these methods cannot satisfy with the requirements of routine clinical diagnosis. Surface-enhanced Raman scattering (SERS)-based immunoassay using functional nanoparticles has attracted more and more attention due to its higher sensitivity. Herein, we report a SERS-based competitive immunoassay for highly sensitive detection of E2. The method can be applied to detect E2 with good precision at concentrations as low as 0.1 pg/mL. The proposed method has been successfully applied to the determination of E2 for 30 human sera and showed a good correlation compared with the commercially available chemiluminescent microparticle immunoassay (CMIA) kit with good reproducibility. This method has exhibited great potential in the clinical analysis of E2 in human serum.