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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Element-based Absolute Quantification using ICP-OES and ICP-MS

2015년 8월 27일 12시 04분 42초
ANAL2-3 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 09시 : 40분
분석화학 - Material Sciences in Analytical Chemistry
저자 및
한국표준과학연구원(KRISS) 분석화학표준센터, Korea
An element-based reductive approach provides an effective means of realizing International System of Units (SI) traceability for high-purity organic and biological standards. In this presentation, KRISS' approach to establish SI-traceable of pure nucleic acids and protein standards will be introduced. For protein stanards, a novel quantification method using double isotope dilution (ID) inductively-coupled plasma mass spectrometry (ICP-MS) combined with microwave-assisted acid digestion was developed for the first time. The method was validated and applied to certify candidate protein reference material (RM) of human growth hormone (hGH). The concentration of hGH was determined by analyzing the total amount of sulfur in hGH. Next, the size-exclusion chromatography method was used with ICP-MS to characterize and quantify sulfur-containing impurities. By subtracting the contribution of sulfur-containing impurities from the total sulfur content in the hGH CRM, SI-traceable certification value was obtained. The quantification result obtained with the present method based on sulfur analysis was in excellent agreement with the result determined via a well-established protein quantification method based on amino acid analysis using conventional acid hydrolysis combined with an ID liquid chromatography-tandem mass spectrometry. The developed method was applied for accurate analysis of protein drugs and standards including hGH, human thyroglobulin (hTG) and insulin. The element-based quantification methods developed here can be generally used for SI-traceable absolute quantification of pure organic- and bio-molecule standards.