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학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Building a Multi-photon Fluorescence Microscope

등록일
2015년 8월 27일 16시 05분 06초
접수번호
0962
발표코드
PHYS.P-259 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 16일 (금요일) 13:00~14:30
발표형식
포스터
발표분야
물리화학
저자 및
공동저자
기영철, 이호재1,*
광주과학기술원(GIST) 화학과, Korea
1광주과학기술원(GIST) 화학, Korea
With few exceptions, biological samples strongly scatter light, prohibiting conventional microscopy from obtaining high spatial-resolution deep tissue images for them. Instead, multi-photon fluorescence microscopy is a useful tool for whole cell imaging and in-vivo tissue imaging, especially neuron because it uses light in near-infrared region that provides deep penetration depth.. Morover, its small excitation volume enables optical sectioning for 3D image reconstruction.In this poster we are reporting the recent progress in our lab of multi-photon microscope build-up. The home-build microscope is composed of a tunable high-power Ti:Sapphire oscillator, galvo scanning mirror, a motorized linear stage for z-axis scanning, and a PMT detector. The apparatus is controlled via custom software written in LabView. Signals are digitized and analyzed in Matlab. It shows lateral and axial resolution of xxx nm and xxx nm, respectively. It takes xx mintues to take an image of 512x512 pixels. Currently we investigate 3D the oxidative stress distribution in a live cell with the probe of ROS generation, CM-H2DCFDA. Three-photon fluorescence image can also be acquired on the same microscope setup. .

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