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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Pepsin immunoassay for the diagnosis of laryngopharyngeal reflux disease

등록일
2015년 8월 27일 16시 08분 38초
접수번호
0974
발표코드
ANAL.P-325 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 16일 (금요일) 13:00~14:30
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
권지윤, 현혜리, 신재호*
광운대학교 화학과, Korea
A number of patients who complain of laryngopharyngeal reflux disease (LPR) is on the gradual increase due to changes in climate, environment, and eating habits. LPR causes hoarseness, frequent throat clearing, bitter taste in the mouth, referred ear pain, chronic cough and even larynx cancer. However, the effective treatment of LPR is difficult because the treatment yet stay in the inhibition of gastric acid secretion as well as the symptoms persist after treatment. Therefore, appropriate treatment through accurate and rapid diagnosis is necessary. Since pepsin is proteolytic enzyme produced only in the stomach, detection techniques of pepsin in saliva is useful as a sensitive and non-invasive method for the reflux of stomach contents. Therefore, pepsin is one of the most important biomarkers to diagnose LPR. Herein, we report on a new approach to design an immunoassay based on the enzyme-linked immunosorbent assay (ELISA). Binding of antigen-antibody converts a colorless substrate to a colored product by using enzyme. Furthermore, to confirm the presence of pepsin in saliva resulting from LPR, we checked the pH of saliva through the color changes of various kinds of indicator. The general pH of saliva is about 6.7, but in the case of saliva which contains pepsin, the pH decreases. We compared the color between different pH of phosphate buffer and artificial saliva in acidic and basic condition of indicators. In this ways, we investigate the utility of colorimetric immunoassay to determine pepsin in biological samples (i.e., saliva).

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