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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Analysis of Phospholipids, Lipid A and Sterols from Outer Membrane Vesicles Shed by Acinetobacter baumannii DU202

2015년 9월 3일 10시 08분 43초
MEDI.P-514 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 15일 (목요일) 11:00~12:30
저자 및
박세민, 방글1, 김영환1,*
한국기초과학지원연구원 질량분석연구동, Korea
1한국기초과학지원연구원 생의학오믹스연구팀, Korea
Acinetobater baumannii DU202 (A. baumannii) is a gram-negative bacterium and well known resistant to various antibiotic drugs as multidrug-resistant bacteria (MDR). Recently, quantitative proteomic analysis of A. baumannii has been reported to cell wall and plasma membrane fractions including outer membrane vesicles (OMV) and outer membranes (OM). The OMVs of gram-negative bacteria are composed mainly of proteins, lipids, lipopolysaccharide (LPS) and sterol. In several cases, they contain either RNA or DNA, and are associated with various biological functions for their growth, development and survival such as immune response systems, secretion and transfer of contents to host cell. Therefore, to comprehend the structural characterization and function of OMV and OM in A. baumannii is important. In this study, we established the analytical method to effectively analyze extracted phospholipids, Lipid A and cholesterol levels from OMV and cell of A. baumannii using UPLC/Q-TOF MSE, MALDI-TOF MS and GC-MS, respectively. Also, to investigate the different biogenesis pathway, novel biological roles of OMV and potential biomarker between OMV and cells, we studied the significant properties and various function of OMV and performed multivariate statistical analysis such as PCA and S-plot model of OPLS-DA.