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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Realization of practical sheet-type biosensor based on multi wall carbon nanotubes and micro-pore filter paper for early detection of prostate cancer

2015년 9월 3일 11시 33분 03초
MAT.P-473 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 16일 (금요일) 13:00~14:30
저자 및
지성경, 이명순, 김 돈*
부경대학교 화학과, Korea
The aim of this study is realization of an inexpensive, simple and sensitive biosensor to detect early stage of prostate cancer using bioactivated-multi wall carbon nanotubes (MWCNTs, diameter 20 nm, length 5 μm) and a micro-pore filter paper (pore size 0.45 μm). For the immunoassay of prostate specific antigen (PSA), which is a biomarker of prostate cancer, carboxylated MWCNTs were activated with PSA antibody (monoclonal antibody of the prostate specific antigen) by using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt (NHSS). The activated MWCNTs were deposited on micro-pore filter paper to use as a bio-sensisensor. The sensor can assay from 0 to 500 ng/mL of PSA level within 2 h with the detection limit of 1.18 ng/mL with an inexpensive bench top digital multimeter (5 1/2 digits). The detection range and sensitivity of the prepared sensor are good enough to diagnose the early stage of prostate cancer (> 4 ng/mL of PSA). This paper-based biosensor is 23 times cheaper (fabricated biosensor price: 2.4 $) and 12 times faster than enzyme-linked immunosorbent assay (ELISA), which is a general method for the detection of a specific protein in modernized hospitals. Furthermore, the maximum detection limit is about 50 times higher than ELISA