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학술발표회초록보기

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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Selective detection and quantification of hOGG1 by real-time PCR

등록일
2015년 9월 3일 14시 08분 49초
접수번호
1233
발표코드
BIO.P-216 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 15일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
김은택, 홍인석*
공주대학교 화학과, Korea
It is important to detect the DNA repair enzymes to understand the role of their activity in our body. Human 8-oxoguanine DNA N-Glycosylase 1 (hOGG1) is an 8-oxoguanine DNA glycosylase which acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic (AP) site. The AP-lyase activity cleaves 3´ to the AP site leaving a 5´ phosphate and a 3´-phospho-α, β-unsaturated aldehyde. Using the activity of hOGG1, we designed the specific single strand DNA (8-oxodG ssDNA), which has a 8-oxoguanine nucleotide (located at the center of sequence) and primer annealing sequences (located at the each terminal sites of sequence). After addition of short complementary ssDNA to the 8-oxodG ssDNA solution, the aliquot of hOGG1 was added to the dsDNA. In the presence of hOGG1, the target DNA was cleaved to the short oligomer, which can not be efficiently amplified by real-time PCR. We monitored the Ct values of amplified DNA in the range of concentration of hOGG1. The Ct values were well correlation to the added amount of hOGG1. This correlation curves can be used to detect hOGG1 in unknown specimen.

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