abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Charaterization of N- and O-linked Glycans from Glycoproteins by MALDI-MS and MS/MS

등록일
2015년 9월 3일 14시 16분 29초
접수번호
1236
발표코드
MEDI.P-515 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 15일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
의약화학
저자 및
공동저자
박인영, 진장미1, 안현주, 김영환2,*
충남대학교 분석과학기술대학원, Korea
1한국기초과학지원연구원 질량분석연구부, Korea
2한국기초과학지원연구원 생의학오믹스연구팀, Korea

Glycosylation is important role in signal transduction between cells, and various biological processes. Biosimilar is a clone of a biotherapeutic products that have been developed by leveraging biotechnology, such as gene recombination and cell culture techniques. Therefore, glycosylation analysis is necessary during various stages of a biotherapeutic product’s lifecycle and one of the most critical tools in the characterization of biotherapeutic proteins. In this study, N-glycans are enzymatically released by N-glycosidase F(PNGase F). O-glycans are chemically released by β-elimination. they are purified by SPE(solid-phase extraction)-PGC. The permethylation of sialylated glycans results in the conversion of all hydroxyl groups into methoxyl groups and serves to render glycans hydrophobic which may increase the signal intensity in mass measurement. We revealed the characterization of N- and O-glycans released from glycoproteins by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and tandem mass spectrometry (MALDI-MS/MS) with 2,5-dihydroxybenzoic acid (2,5-DHB) as the matrix.


상단으로