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제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Analysis of fluorescence-labelled starch using asymmetrical flow field-flow fractionation (AsFlFFF) with multiple detectors

등록일
2015년 9월 3일 15시 50분 32초
접수번호
1273
발표코드
ANAL.P-341 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 16일 (금요일) 13:00~14:30
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
유영석, 이승호*, 최재영, Haiyang Dou1, Claudia Zielke2, Lars Nilsson3
한남대학교 화학과, Korea
1Hebei University, China, China
2Lund University, Sweden, Germany
3Lund University, Sweden, Sweden
※ 국외소속으로 등록된 저자의 승인여부는 최소 3일이내 발표자 email로 알려드립니다.
승인 3건
Starch is a mixture of amylose (AMY) and amylopectin (AMP). AMY molecules are essentially linear, while AMP molecules have branches with 5-6% alpha-(1,6) linkages. Usually AMY and AMP are different in physical properties such as molar mass, radius of gyration (Rg) and hydrodynamic radius (Rh). The rheological and functional properties of starch are influenced by various factors including the molecular size, molar mass distribution and the concentration ratio of AMY to AMP. It is also important to analyze proteinaceous material in starch for characterization of starch. In this study, asymmetrical flow field-flow fractionation (AsFlFFF) was employed for separation and quantitation of AMY and AMP. AsFlFFF was coupled with multiple detectors such as multi-angle light scattering (MALS) and refractive index (RI) detectors for determination of absolute molar mass, molar mass distribution, and molecular structure without the need for system calibration. And then, for analysis of proteinaceous matter in starch, starches were fluorescence-labelled, and then analyzed by AsFlFFF coupled with a fluorescence detector.

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