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학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Global landscape of miRNA target sites reveals regulation of the Wnt pathway by miRNA in human embryonic stem cells

등록일
2015년 9월 4일 15시 10분 58초
접수번호
1325
발표코드
BIO.P-218 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 15일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
권영수
세종대학교 바이오융합공학과, Korea
miRNAs in association with AGO2 protein bind partially complementary target sites on mRNAs to regulate target mRNA expression. A vast number of mRNAs harbor potential miRNA target sites, yet most of mRNAs are still devoid of experimental evidence for direct binding of AGO proteins. To address this issue, we generated a knock-in human embryonic stem cell (hESC) line that expresses flag-tagged AGO2 protein from its native genomic locus and carried out crosslinking-immunoprecipitation coupled with next-generation sequencing (CLIP-seq) for AGO2 with an unprecedented sensitivity and specificity. Consistent with the generally accepted model, complementarity between mRNA and 5' nucleotides 2 to 7 or 8 of miRNA were an important determinant for AGO2 binding. In this study, I identified known and novel targets of miRNAs. Unexpectedly, a large number of genes in canonical and non-canonical Wnt pathways were targets of miRNAs in hESCs. The data presented here may provide a framework for computational and biological studies on miRNA functions.

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