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제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Displacement phenomena in Serial Affinity Column Set (SACS)

등록일
2015년 9월 6일 20시 26분 37초
접수번호
1338
발표코드
ANAL.P-347 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 16일 (금요일) 13:00~14:30
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
윤민규, 조원련*
원광대학교 바이오나노화학부, Korea
The work described here examines displacement phenomena that play a role in lectin affinity chromatography and their potential to impact reproducibility. This was achieved using Lycopersicon esculentum lectin (LEL), a lectin widely used in monitoring cancer. Four small LEL columns were coupled in series to form a single affinity chromatography system. The serial affinity column set (SACS) was then loaded with human plasma proteins. At the completion of loading, the column set was disassembled, the four columns eluted individually, the captured proteins trypsin digested, the peptides deglycosylated with PNGase F, and the parent proteins identified through mass spectral analyses. Significantly different sets of glycoproteins were selected by each column, some proteins appearing to be exclusively bound to the first column while others were bound further along in the series. Clearly sample displacement chromatography (SDC) occurs. Glycoproteins were bound at different places in the column train; identifying the presence of glycoforms of different affinity on a single glycoprotein.

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