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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Highly sensitive SERS-based immunoassay as an early diagnostic tool in rheumatoid arthritis [우수포스터상]

2015년 9월 7일 14시 27분 14초
ANAL.P-348 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 16일 (금요일) 13:00~14:30
저자 및
전향아, 주재범1,*
한양대학교 바이오나노공학과, Korea
1한양대학교 생명나노공학과, Korea
Rheumatoid arthritis is both common and chronic, with significant consequences for multiple organ systems. The key to therapeutic success lies in identifying individuals who will have severe destructive disease as early as possible, so that effective treatment can be initiated before irreversible damage occurs. Anti-cyclic citrullinated peptide (anti-CCP) antibody is well known biomarker as a particularly useful biomarker in the diagnosis of RA, with high specificity, presence early in the disease process, and ability to identify patients who are likely to have severe disease and irreversible damage. In this study, we assessed the clinical feasibility of conducting immunoassay based on surface-enhanced Raman scattering (SERS). An anti-CCP was used as a biomarker, magnetic beads conjugated with CCP were used as substrates, and the SERS nano-tags were comprised of anti-human IgG-conjugated hollow gold nanospheres (HGNs). We were able to determine the anti-CCP serum levels successfully by observing the distinctive Raman intensities corresponding to the SERS nano-tags. At high concentrations of anti-CCP (>25 U/mL), the results obtained from the SERS assay confirmed to those obtained via an ELISA-based assay. Nevertheless, quantitation via our SERS-based assay is significantly more accurate at low concentrations (<25 U/mL). For this study, 74 clinical blood samples were tested and we compared the quantitative results of an anti-CCP obtained from the SERS-based assay to that of a commercial ELISA kit.