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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Development of SERS imaging-based lateral flow immunosensor for rapid and sensitive detection of food toxin

2015년 9월 7일 14시 51분 56초
ANAL.P-349 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 16일 (금요일) 13:00~14:30
저자 및
황준기, 주재범1,*
한양대학교 바이오나노학과, Korea
1한양대학교 생명나노공학과, Korea
Lateral flow immunoassay (LFIA) is uncomplicated and intended assay method to detect the presence of an antigen in sample without the need for specialized or costly equipment. However, some of the problems associated with low sensitivity and limitations in quantitative analysis have made this technique less attractive. Here, we developed a surface-enhanced Raman scattering (SERS) imaging-based lateral flow immunosensor to overcome the limitation of conventional LFIA kit. In SERS imaging-based LFIA strip sensor, all the measurement principle is same with conventional LFIA strips except detection nanoprobes. The Raman-reporter labelled hollow gold nanosphere (HGN) was used as a SERS nanoprobe instead of gold nanospheres used in conventional LFIA strips. To verify the feasibility of the devised SERS imaging-based LFA strip platform, an immunoassay of staphylococcal enterotoxin B (SEB) was performed as a model reaction. The presence of SEB can be identified through the color change of the test zone. In addition, a highly precise quantitative evaluation of SEB is possible by averaging the SERS mapping signals. The sensitivity of proposed LFIA sensor was compared with contrast imaging analysis of test zone and conventional ELISA method. The LOD of the proposed LFIA sensor was estimated to be 1 pg/mL, and this low LOD value demonstrates that our SERS imaging-based LFIA immunosensor is approximately three orders of magnitude more sensitive than corresponding control methods. Accordingly, our SERS-based LFIA strip sensor shows significant potential for the rapid and sensitive detection of target markers in a simple manner.