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09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능
제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내
Time-dependent Metabolomics’ Analysis of Traditional Nuruk Starters with Different Raw Materials and Water Content During Fermentation
2015년 9월 8일 15시 08분 32초
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10월 15일 (목요일) 11:00~12:30
전북대학교 생리활성물질연구소, 생리활성소재과학과, Korea
전북대학교 생리활성소재과학과, Korea
As our ongoing work for the investigation of the metabolomics of traditional Nuruk starter fermentation in the time-dependent manner, the preliminary work was performed on Nuruk starter (named as TN-1 and 2) prepared from whole wheat as an raw material, and the primary metabolites including sugars and sugar alcohols, amino acids, several organic acids and fatty acids, were analyzed in terms of the variation of kind and the amounts of metabolites. In the present poster, as a further work, the effects of the raw materials and the water contents of traditional Nuruk starter at initial preparation step on the metabolomics were investigated and compared with the primary data obtained from the fermentation of whole wheat Nuruk starter. The raw materials used in preparation of Nuruk disks were barley, green gram, and whole wheat, and two types of composition were prepared, i.e. the one is composed of barley and green gram (3.3:0.52, by weight, named as TN-D), the one is composed of barley, green gram, and whole wheat (3.3:0.52:3.3 by weight, named as TN-C). The water contents on preparation were maintained as 20, 26, and 30%. The Nuruk disks were incubated at 36 oC under 70% of humidity for 30 days. The time-dependent Nuruk samples were collected at 0, 3rd, 6th, 10th, 20th, 30th day after start of fermentation. All samples were chemically modified via oximation and subsequent in-situ silylation, and analyzed with GC-MS. The general features is that the metabolites observed in each Nuruk starters were same in their kinds, and this was shown to be same as results of our preliminary work with whole wheat Nuruk starter. Comparing variation in amounts of organic acids, amino acids with the laps of time metabolites, in TN-C the hyperbolic curve pattern was observed with two maxima at 6 and 20 days. The higher the water content was, the large amount of metabolite was. In sugars, most of sugars including xylose and fructose were following same pattern, but sucrose and maltose showed no definite tendency like other sugars. In sugar alcohols such as glycerol and arabitol were shown similar pattern to sugars, but the amounts xylitol and mannitol increased to maximum at 20 days and decreased after that. The amounts of fatty acids increased continuously in time-dependent manner and reached maximum at 20 days. In TN-D, however, that kind of pattern observed in TN-C was not observed clearly, and it was notable that the water content did not show any correlation with the variation of metabolites in amounts. The observation in TN-C was similar to TN-1 and 2.
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