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학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 An alternative mode of gene regulation by microRNAs

등록일
2015년 9월 8일 16시 49분 16초
접수번호
1404
발표코드
BIO.P-225 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 15일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
권영수
세종대학교 바이오융합공학과, Korea
MicroRNAs(miRNA) in association with Argonaute (AGO) family proteins regulate gene expression by repressing translation or reducing mRNA stability. There are 4 different AGO proteins in humans and these proteins are largely equivalent in gene regulation function. Interestingly, endonuclease activity of Argonaute protein in animals are well conserved although the activity is apparently not required for miRNA-mediated gene regulation. In mammals, only AGO2 has endonuclease activity and it was demonstrated that nucleolytic activity is essential for perinatal viability in mice. Although it was shown that the activity is essential for biogenesis of miR-451, it is questionable that for biogenesis of a single miRNA species catalytic activity of AGO2 is conserved in vertebrates. Here, I present an alternative mode of gene regulation by miRNA that requires catalytic activity of AGO2. Based on AGO2-CLIP-seq data, we identified target sites were cleaved by AGO2 in a miRNA-dependent manner. miRNA-dependent endonucleolytic cleavage does not require extensive base pairing like siRNA-mediated cleavage. This finding provides a novel mechanism of miRNA-mediated gene regulation. Further studies are needed for biological and pathological relevance of gene regulation by target cleavage.

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