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제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Imaging Mass Microscopy for high spatial resolution analysis

2015년 9월 9일 16시 32분 19초
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목 13시 : 50분
분석화학 - Diverse Technologies in Spectroscopy - Joint Symposium with JAIMA
저자 및
Koretsugu Ogata
Shimadzu Corporation, Japan, Korea
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승인 1건
Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of molecules and drugs in organs. In particular, iMScope TRIO is an "imaging mass microscope" instrument that is equipped with an optical microscopy, an atmospheric pressure ion-source chamber for MALDI and a quadrupole ion trap time-of-flight (QIT-TOF) analyzer. This instrument is capable of narrowing down the laser diameter to minimum of 5 μm for high spatial resolution MS imaging.
We performed IMS using that dosed tissue sections were surgically removed from mouse liver after treatment with drugs. Samples were frozen in dry ice acetone and, then sectioned at a thickness of 10 μm and transferred to an ITO slide glass (Sigma).
We succeeded the MS and MS/MS imaging performed with high spatial resolution (5 μm). Compared to the result acquired at 25 μm resolution, the number of positions that gave signal appears smaller at higher resolution. On magnification view of the overlaid image, revealed that the positions of detected signals were localized to narrow intercellular spaces. This result suggests that drug accumulates outside of cells or on cell membrane.
In conclusion, iMScope TRIO demonstrated a clear picture of drug imaging which showed that the target molecule is presumably localized on cell membrane or in intercellular spaces and not contained within the hepatocytes. Such observation was only possible at 5 μm spatial resolution. This level of spatial resolution achieved is indispensable for unraveling the physiology of pharmacokinetics at cellular level. Moreover, the above observation was only noticeable after superimposing the MS/MS image onto the optical image. The optical microscope incorporated in the instrument is giving iMScopeTRIO its unique and important features that magnification and resolution of optical imaging is high.