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학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Imaging Mass Microscopy for high spatial resolution analysis

등록일
2015년 9월 9일 16시 32분 19초
접수번호
1409
발표코드
ANAL1-3 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 13시 : 50분
발표형식
심포지엄
발표분야
분석화학 - Diverse Technologies in Spectroscopy - Joint Symposium with JAIMA
저자 및
공동저자
Koretsugu Ogata
Shimadzu Corporation, Japan, Korea
※ 국외소속으로 등록된 저자의 승인여부는 최소 3일이내 발표자 email로 알려드립니다.
승인 1건
Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of molecules and drugs in organs. In particular, iMScope TRIO is an "imaging mass microscope" instrument that is equipped with an optical microscopy, an atmospheric pressure ion-source chamber for MALDI and a quadrupole ion trap time-of-flight (QIT-TOF) analyzer. This instrument is capable of narrowing down the laser diameter to minimum of 5 μm for high spatial resolution MS imaging.
We performed IMS using that dosed tissue sections were surgically removed from mouse liver after treatment with drugs. Samples were frozen in dry ice acetone and, then sectioned at a thickness of 10 μm and transferred to an ITO slide glass (Sigma).
We succeeded the MS and MS/MS imaging performed with high spatial resolution (5 μm). Compared to the result acquired at 25 μm resolution, the number of positions that gave signal appears smaller at higher resolution. On magnification view of the overlaid image, revealed that the positions of detected signals were localized to narrow intercellular spaces. This result suggests that drug accumulates outside of cells or on cell membrane.
In conclusion, iMScope TRIO demonstrated a clear picture of drug imaging which showed that the target molecule is presumably localized on cell membrane or in intercellular spaces and not contained within the hepatocytes. Such observation was only possible at 5 μm spatial resolution. This level of spatial resolution achieved is indispensable for unraveling the physiology of pharmacokinetics at cellular level. Moreover, the above observation was only noticeable after superimposing the MS/MS image onto the optical image. The optical microscope incorporated in the instrument is giving iMScopeTRIO its unique and important features that magnification and resolution of optical imaging is high.

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