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학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Detection of E. coli O157:H7 by ICP-MS immunoassay using metal-doped magnetic nanoparticles

등록일
2015년 8월 27일 10시 24분 36초
접수번호
2009
발표코드
ANAL1.O-9 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 09시 : 32분
발표형식
구두발표
발표분야
분석화학 - Oral Presentation of Young Analytical Chemists I
저자 및
공동저자
안효정, 임흥빈*
단국대학교 화학과, Korea
Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) is a major food-borne pathogen causing severe disease in humans worldwide. E. coli O157:H7 is a pathogenic bacterium capable of contaminating food, causing food poisoning, bloody diarrhea, and occasionally kidney failure. For the detection, ICP-MS immunoassay employing magnetophoretic chromatography separation technique was to be used in this work. For target extraction and detection, amine-functionalized/Cs-doped multi-core magnetic nanoparticles (MNPs) were synthesized. The extraction is based on the highly bio-specific recognition interactions of antibody?antigen, i.e, antibody-conjugated MNPs captured E. coli O157:H7. The unbound excess MNPs were eliminated by magnetic separation in the presence of polyethylenglycol medium. The resultant immunoassay products were effectively extracted from sample solution by magnetic separation and then quantified by the determination of doped metal detection using Inductively Coupled Plasma Mass Spectrometer(ICP-MS). Since the proposed method reduced sample treatment time and amplify the signal, its application can be wide for various cell detection.

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