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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A rapid and sensitive profiling of free fatty acids in biological samples by liquid chromatography coupled to tandem mass spectrometry with derivatization

2015년 8월 27일 10시 55분 30초
ANAL1.O-12 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 09시 : 44분
분석화학 - Oral Presentation of Young Analytical Chemists I
저자 및
목혁준, 김광표1,*
경희대학교 화학과, Korea
1경희대학교 응용화학과, Korea
Free fatty acids (FFAs) that have roles in cellular energy and signaling are critical molecules in various biological states. Due to the poor ionization efficiency of FFAs under electrospray ionization mass spectrometry (ESI-MS), it is challenging to construct a robust platform to profile various FFAs in biological samples with LC-MS. In this study, we applied trimethylsilyldiazomethane (TMSD) derivatization to improve the sensitivity of FFA profiling by increasing ionization efficiencies. Multiple reaction monitoring (MRM) was used for the selective quantification of methylated FFAs. The performance of optimized TMSD methylation was validated for a reliable FFA profiling. By using liquid chromatography (LC)-ESI-MS, the high-throughput analysis of various FFAs were successfully carried out in 5 min. In order to prove the utility of our developed method, we performed the FFA profiling of mouse brain, liver, and plasma by using LC/MS/MS with/without methylation. As a result, comparing to nonmethylation, various FFAs were successfully analyzed by using methylation derivatization. Strikingly, we can successfully identify and quantify 14, 14 and 9 FFAs from mouse liver, brain and plasma among the 29 targeted FFAs.