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현재 가능한 작업은 아래와 같습니다.
  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 High Sensitive Analytical Platform for Glycolipids Characterization from Cells to Tissues using Negative Nano-LC/MS and LC/MS/MS

2015년 8월 27일 11시 43분 41초
ANAL1.O-13 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 09시 : 48분
분석화학 - Oral Presentation of Young Analytical Chemists I
저자 및
황재윤, 안현주*
충남대학교 분석과학기술대학원, Korea
Gangliosides are glycosphingolipids containing one or more sialic acids. They participate in diverse biological processes including cell-cell recognition and the modulation of membrane protein function. In spite of the biological importance of cell surface gangliosides, the studies for gangliosides are being delayed due to the structural complexity, amphipathic nature, and lack of sensitive analytical method. Here, we introduces a robust analytical platform for the rapid profiling and quantitation of gangliosides on cell surface using membrane enrichment method and negative ion-mode nano-LC chip based mass spectrometry. The analysis of gangliosides using high resolution mass spectrometry with a membrane enrichment method provides not only comprehensive profile on cell surface gangliosides but also confident assignment of chain length and the degree of unsaturation of ceramide. We optimized the conditions such as solvents, gradients, and sample concentration to efficiently separate ganglioside standard in negative ion-mode. For profiling of cell surface gangliosides, cell membranes were isolated and enriched by ultracentrifugation. Membranes were further separated by Folch partitioning and then fractionated by C18 SPE to extract gangliosides. Gangliosides were comprehensively profiled by negative ion mode LC/MS and the structures were elucidated by tandem MS. We successfully explored cell surface gangliosides in MCF7 breast cancer cell and obtained over 70 compositions. GM3, GM2, GM1 and GD1 were commonly observed as major gangliosides showing various ceramide portions.