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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제116회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Top-down Mass Spectrometry to Monitor Thrapeutic Protein with Glycan Heterogeneity

2015년 8월 27일 13시 56분 16초
ANAL1.O-19 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 10시 : 12분
분석화학 - Oral Presentation of Young Analytical Chemists I
저자 및
김현중, 서영숙, 안현주*
충남대학교 분석과학기술대학원, Korea
Biotherapeutic glycoproteins including recombinant monoclonal antibody(mAb) and Erythropoietin (EPO) are fast-growing sector in the current pharmaceutical market. They play a major role in the treatment of variety of conditions such as cancer, inflammation, auto-immune disease for mAb and anemia resulting from chronic kidney disease and inflammatory bowel disease for EPO. It is well known that glycosylation of therapeutic glycoprotein correlates with product safety, efficacy and quality. Thus it has become important to characterize its composition and molecular heterogeneity. In this study, we have developed a top-down approach using chip-based C8 nano-LC/Q-TOF MS. MS parameter was optimized such as gradient, flow rate, fragment voltage to get the best performance. The top-down approach should be preferable in the field of biopharmaceutical as it is relatively fast and more simplified sample preparation procedure. MAbs having neutral glycans and EPOs containing highly sialylated glycans are used to show the proof of concept for the method. We could directly monitor the multiple charge-state distribution and identified the presence and abundance of different glycoform with low nanogram levels of intact glycoprotein. The results can be applied to quality control evaluator and production of biotherapeutic glycoprotein. Especially method can satisfy the need for evaluating the similarity of the glycosylation profiles between biosimilar and innovator products.