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  • 09월 08일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

대한화학회 제116회 학술발표회 및 총회 Highly sensitive detection of HIV-1 DNA using SERS-based lateral flow immunosensor

등록일
2015년 9월 7일 16시 36분 51초
접수번호
2126
발표코드
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발표시간
금 16시 : 10분
발표형식
구두발표
발표분야
분석화학 - Oral Presentation of Young Analytical Chemists II
저자 및
공동저자
유지민, 주재범1,*
한양대학교 바이오나노학과, Korea
1한양대학교 생명나노공학과, Korea
We report a surface-enhanced Raman scattering (SERS)-based lateral flow assay method for the highly sensitive detection of target nucleic acids. Human immunodeficiency virus type 1 (HIV-1) DNA was used as a model analyte in this work. Here, Raman reporter (Malachite green isothiocyanate (MGITC)) and detection-DNA co-functionalized gold nanoparticles (AuNPs) were employed as SERS nano tags for targeting specific nucleic acids. In the presence of HIV-1 DNA, the sandwich type “detection DNA-conjugated SERS tag - target DNA - capture DNA” hybridization reactions occurred and the SERS tags induced strong Raman intensities on the test line. On the contrary, without HIV-1 DNA, the SERS tags could not be captured on the surface of test line, resulting in very low or no SERS intensities. With the increase of HIV-1 DNA concentration, the SERS intensities on the test line were gradually increased. The characteristic Raman scattering peak intensity of MGITC was measured for the quantitative detection of HIV-1 DNA. Under optimized conditions, the minimum detectable concentration for our SERS-based lateral flow assay was 0.5 pM, which was improved about 200-fold compared with the colorimetric detection approach or fluorescent method. This result demonstrate the potential feasibility of SERS-based lateral flow assay to detect a broad range of genetic marker for in-field or POC diagnostics.

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