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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제117회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Genome editing via CRISPR-Cas9 nucleases

등록일
2016년 2월 4일 02시 21분 49초
접수번호
0193
발표코드
BIO2-4 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 25분
발표형식
심포지엄
발표분야
생명화학 - 유전체 교정과 생명화학 I (Genome Editing and Life Chemistry I)
저자 및
공동저자
배상수
한양대학교 화학과, Korea
Genome editing with engineered nucleases such as ZFNs (zinc finger nucleases), TALENs (transcription-activator-like effector nucleases), and CRISPR/Cas-derived RNA-guided endo nucleases (RGENs) is broadly useful for biomedical research, biotechnology, and medicine. Unlike ZFNs and TALENs whose DNA specificities are determined by DNA-binding proteins, RGENs use complementary base pairing to recognize target sites. Custom-designed RGENs are produced simply by replacing guide RNAs, making this system easy to access. Unfortunately, RGENs cleave not only on-target sites but also off-target sites that differ by up to several nucleotides from the on-target sites, causing unwanted off-target mutations and chromosomal rearrangements. Furthermore, these nucleases often induce in-frame mutations in target genes, reducing the efficacy of nucleases in a population of cells and hampering the isolation of biallelic null clones. Here I present a novel potential off-target searching tool and a Microhomology-predictor for inducing more efficient knock out. Furthermore, I introduce a novel genome-wide profiling method of CRISPR-Cas9 off-target effects in human cells. These tools are indispensable for carrying out the genome-wide knock-out screening or gene mutation in human iPS cells, animals and plants.

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