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제117회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Enhanced mercury(II) reduction by Shewanella oneidensis MR-1 using flavin as an electron shuttle

등록일
2016년 2월 11일 11시 37분 50초
접수번호
0345
발표코드
ENVR.P-496 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 22일 (금요일) 13:00~14:30
발표형식
포스터
발표분야
환경에너지
저자 및
공동저자
QASIM GHULAM HUSSAIN, 한승희*
광주과학기술원(GIST) 환경공학과, Korea
Shewanella oneidensis MR-1, a dissimilatory metal reducing bacterium, has been shown to reduce inorganic divalent mercury [Hg(II)] at low Hg(II) concentrations (< 0.3 μM). However the electron transport process for Hg(II) reduction during anaerobic respiration of MR-1 was unclear. MR-1 secretes flavin mononucleotide (FMN) and riboflavin for extracellular electron transfer. Using riboflavin as a redox mediator, the role of endogenous and exogenous electron shuttle for Hg(II) reduction by MR-1 was investigated. Highest specific activity was observed in the presence of riboflavin and Fe(III) (0.35±0.01 pmole Hg0 min-1 mg-1 protein). As riboflavin was removed from media, specific activity declined to 0.06±0.03 (pmole Hg0 min-1 mg-1 protein). Further investigation revealed that Hg(II) reduction rate by wild type strain is higher than that of Δbfe (mutant lacking the ability to export flavin) (0.13 vs 0.06 h-1). Increased Hg(II) reduction rate by wild type strain than Δbfe is attributed to electron shuttling of flavin. The results demonstrate that, using flavin as an electron shuttle, MR-1 can effectively reduce Hg(II). This electron shuttling could be an important process of mercury detoxification in subsurface environments where dissolution of humic substances is limited.

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